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| Cell line: | SW-480 |
| DSMZ no.: | ACC 313 |
| Species: | human (Homo sapiens) |
| Cell type: | colon adenocarcinoma |
| Origin: | established from the tumor of a 50-year-old man with colon adenocarcinoma (grade 4, Duke class B) |
| Reference(s): | 14713 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | endothelial-like adherent cells, mostly bipolar with microvilli growing in monolayers; image; image; image; image; image |
| Medium: | 90-95% RPMI 1640 + 5-10% h.i. FBS |
| Subculture: | seed out at about 0.5-1.0 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | 25-30 hours |
| Harvest: | cell harvest of about 10-15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotriploid karyotype with 12% polyploidy - 58(51-58)<3n>XX, -Y, -1, -3, -4, -5, -6, +7, -8, -10, -12, -15, -18, -19, +20, -22, der(1)t(1;9)(q22;q12), t(2;12)(q35;q13.1), del(5)(q13.2q32), add(7)(q35), dup(7)(q11.22q21.3), der(8)t(1;8)(p11;p11)t(1;9)(q22;q12), add(10)(p14), inv(14)(q13q32), del(18)(q12.2), der(19)t(6;19)(p11;q13), der(20)t(14;20)(q13;p13)x2 - identified by large submetacentric der(2)t(2;12) marker |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
