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| Cell line: | T-24 |
| DSMZ no.: | ACC 376 |
| Species: | human (Homo sapiens) |
| Cell type: | urinary bladder carcinoma |
| Origin: | established from the primary tumor of an 81-year-old woman with urinary bladder carcinoma (transitional cell carcinoma, grade III) in 1970; widely used and extensively characterized cell line (see cellosaurus: Expasy); listed in the CCLE (ref 18232);
produces a variety of cytokines (e.g. G-CSF, IL-6 and SCF) and described to carry HRAS and p53 mutation(s) |
| Reference(s): | 14410, 14409, 15063, 15223, 18168, 14579, 18232, 40404 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid adherent cells growing in monolayers; image; image; image |
| Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture up to 1:5 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5-10% CO2 |
| Doubling time: | ca. 48 hours |
| Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypertriploid karyotype with 8% polyploidy - 70-75<3n>XX, -X, +7, -10, -16, -17, -18, +20, +22, +4mar, add(1)(q11), add(1)(p34), del(6)(q21), del(8)(p21), del(9)(p21), del(10)(p11), add(11)(p13), del(13)(q13q14), i(14q), der(15q21q), add(18)(p11) - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
