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| Cell line: | T-47D |
| DSMZ no.: | ACC 739 |
| Species: | human (Homo sapiens) |
| Cell type: | breast carcinoma |
| Origin: | established from the pleural effusion of a 54-year-old woman with infiltrating ductal carcinoma of the breast in 1974; cells were described in the literature to highly express the Wnt-7b oncogene, and positive for the estrogen and progesterone receptor and to express low amounts of androgene and glucocorticoid receptor; RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 17267, 17268, 17269, 18232, 18234, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial cells growing adherently as monolayer; image; image; image; image; image |
| Medium: | 85% RPMI 1640 + 15% h.i. FBS + 10 µg/ml human insulin |
| Subculture: | seed out at ca. 2-4 x 106 cells/175cm2 flask; split confluent culture 1:3 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | expression of ER, PR, AR and HER2 confirmed by Western blot |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
