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| Cell line: | TK-1B |
| DSMZ no.: | ACC 946 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | TK-1B is a subclone of TK-1, obtained by limited dilution. Cell line TK-1 was established in 1984 from the peripheral blood of a 22-year-old man, diagnosed with T-lymphoblastic lymphoma followed by leukemic conversion into acute myeloid leukemia (AML M4). Myelomonocytic characteristics of TK-1B were described to be similar to TK-1; the karyotype is provided in the original paper |
| Reference(s): | 40664, 40665, 15031 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly and partly in small clumps in suspension ; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/ml in 24-well plates with 20% FBS; no cell growth during first week. Later, split culture 1:2 to 1:3 every 2-3 days; maintain at ca. 0.5-1 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48-72 hours |
| Harvest: | cell harvest of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD2-, CD3-, CD4+, CD5-, CD7+, CD8-, CD10+, CD13+, CD14-, CD15+, CD19-, CD20-, CD33+, CD34-, HLA-DR+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
