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| Cell line: | TLBR-1 |
| DSMZ no.: | ACC 904 |
| Species: | human (Homo sapiens) |
| Cell type: | anaplastic large cell lymphoma |
| Origin: | established from primary tumor tissue of a breast implant-associated T-cell anaplastic large cell lymphoma of a 42-year-old-woman; described to carry a mutation in the STAT3 gene (S614R) |
| Reference(s): | 40178, 40179, 40182, 40183 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | most round cells growing singly and in aggregates in suspension; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 0.5 x 106 cells/ml; maintain at 0.3-0.8 x 106 cells/ml; split 1:2 to 1:4 every 2-3 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 30-40 hours |
| Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD2+, CD3-, CD4-, CD5-, CD6-, CD7-, CD8+, CD10-, CD13-, CD14-, CD15+, CD19-, CD20(+), CD21-,
CD25+, CD30+, CD33(+), CD34-, CD71+, CD80+, HLA-DR+, TCRalpha/beta-, TCRgamma/delta-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | absence of fusion gene NPM1-ALK confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
