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| Cell line: | TN-368 |
| DSMZ no.: | ACC 177 |
| Species: | insect - Tiger moth (Trichoplusia ni) |
| Cell type: | larval cells |
| Origin: | established from the larvae of a tiger moth (species Trichoplusia ni = Autographa brassicae = Plusia ni, family Noctuidae, subfamily Plusiinae, order Lepidoptera) |
| Reference(s): | 14609 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | spindle-shaped cells growing in suspension (90%); cells tend to cluster in aggregates; image; image; image; image; image |
| Medium: | 80% TC 100 medium + 20% h.i. FBS |
| Subculture: | split saturated culture 1:4 to 1:10 every 2-3; maintain at 0.4-0.5 x 106 cells/ml; after thawing, cells do not grow during the first 2-4 days and viability may drop to about 10%; seed out at ca. 0.1 x 106 cells/ml |
| Incubation: | without CO2 at room temperature (20-25 °C) |
| Doubling time: | about 24 hours |
| Harvest: | cell harvest of about 0.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Trichoplusia ni species. |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
