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Cell line: | TT2609-A02 |
DSMZ no.: | ACC 859 |
Species: | human (Homo sapiens) |
Cell type: | follicular thyroid carcinoma |
Origin: | established in 1996 from the tumor tissue of a 57-year-old man with local recurrence of follicular thyroid cancer (containing well-differentiated with dispersed poorly differentiated areas). Cells express the transcriptionally inactive (dominant negativ) delta-p53 isoform which is additionally mutated resulting in a truncated p53 form and a short 5 KDA fragment (delta p53 -/-). A sister cell line with an untruncated delta-p53 is available (ACC 510). Useful for studies with p53 family members p63 and p73 |
Reference(s): | 14552 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently in monolayers |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS + 1 mM sodium pyruvate + 1x IST (5 µg/ml insulin + 5 µg/ml transferrin + 5 ng/ml sodium selenite) |
Subculture: | seed out at about 1 x 106 cells/25cm2; split 1:2 to 1:3 once a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 100 hours |
Harvest: | cell harvest of ca. 15 x 106 cells/175 cm2 flask |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: HBV -, HCV -, HIV-1 -, HIV-2 -, EBV -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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