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Cell line: | U-266 |
DSMZ no.: | ACC 9 |
Species: | human (Homo sapiens) |
Cell type: | multiple myeloma |
Origin: | established from the peripheral blood of a 53-year-old man with IgE-secreting myeloma (refractory, terminal) in 1968; cells were described in the literature to produce IgE lambda; possible fusion partner for hybridoma production; cells express mRNA for BCL2 gene. Exome and RNA sequence data are available (see Ref 18187 and Exome sequence and RNA-Seq) |
Reference(s): | 14805, 14486, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round to polygonal, single or clustered cells in suspension, some loosely adherent; image; image; image; image; |
Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.5-0.7 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-3 days; maintain at 0.2-1.0 x 106 cells/ml; cells grow partially (loosely) adherent and can be removed with a bent Pasteur pipette (trypsin is not necessary) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 55 hours |
Harvest: | saturation density at 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, and continuously in PCR assays |
Immunology: | CD3 -, CD5 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 (+), CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, smlambda -, cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human complex hypodiploid karyotype with 6.5% polyploidy - 44(40-46)<2n>XY, -8, -10, -13, -15, +2mar, t(1;11)(p33;q13), add(3)(q27), t(4;11)(q?21;q23), add(7)(q32), add(8)(q24), add(9)(q34), add(10)(p14), add(14)(p11), add(17)(p11), add(18)(p12), der(22)t(15;22)(q21;q13) - 11q13 breakpoint recurrent in multiple myeloma |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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