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Cell line: | U-698-M |
DSMZ no.: | ACC 4 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established from the involved tonsil of a 7-year-old boy with lymphoblastic lymphoma at diagnosis prior to therapy in 1972 |
Reference(s): | 14806, 14807, 15230 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single or clumped cells in suspension; some cells adhere to plastic; image
; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at 0.2-0.7 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; some cells adhere to plastic, but can be detached mechanically (trypsin not necessary) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | maximal density at 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 5.5% polyploidy - 49(44-50)<2n>XY, +3, +7, -14, +mar, dup(1)(q43q21.2), der(2)t(2;3)(p16;p11), add(3)(p11), del(6)(q15q22), del(9)(p22), dup(11)(q23q13), add(13)(p12), add(16)(q24) - carries large submetacentric dup(1) marker |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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