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Cell line: | U2OS#18-XRCC2-5E |
DSMZ no.: | ACC 836 |
Species: | human (Homo sapiens) |
Cell type: | osteosarcoma |
Origin: | Genetical modification of U2OS#18 (DSMZ ACC 832, a reporter cell line for measurement of sister chromatid recombination, SCR) with a genetic disruption of XRCC2 by frameshifting mutations in exon 3 (targeted by
CRISPR-CAS9; puromycin resistant and expressing GFP). The wild-type allele is no longer present. A sister cell line with the retrovirally reconstituted XRCC2 gene is available as ACC 841 |
Reference(s): | 18169, 18205, 18186 |
Biosafety level: | 1, GMO-S1 |
Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
Permissions and restrictions: | A, D |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently as monolayer; image ; image |
Medium: | 80% DMEM + 20% h.i. FBS + (additionally) 2 mM L-glutamine (to verify puromycin resistance, 1 µg/ml should be added) |
Subculture: | seed out at 1 x 106 cells/80 cm2;
split confluent culture once a week 1:2 to 1:3 using trypsin/EDTA (wash twice with PBS before adding trypsin/EDTA); apparently confluent cultures can be further cultivated for 4-6 days to increase the maximal cell harvest |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 4-6 days |
Harvest: | cell harvest of ca. 4-8 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | frameshift mutations in exon 3 of XRCC2 were verified by sequence analysis |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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