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| Cell line: | UCSD-AML1 |
| DSMZ no.: | ACC 691 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myeloid leukemia |
| Origin: | established from the bone marrow of a 73-year-old woman with acute myeloid leukemia at relapse in 1989; cells were described in the literature to be cytokine-dependent (GM-CSF, IL-6, or M-CSF) and to carry the translocations t(3;3)(q21;q26) and t(12;22)(p13;q12) leading to MECOM (EVI1) overexpression and to ETV6-MN1 (TEL-MN1) fusion gene, respectively |
| Reference(s): | 16052, 16053 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polygonal single cells with variable sizes in suspension, a few cells are lightly adherent; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml GM-CSF |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.2-1.0 x 106 cells/ml; split culture 1:3 to 1:5 every 3-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | maximum density at ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD4+, CD13(+), CD14-, CD15-, CD19-, CD33+, CD34+, HLA-DR(+), cyCD3-, cyCD68(+); image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypodiploid karyotype with 10% polyploidy; 45(42-45)<2n>XX,-7, t(3;3)(q21;q26), t(12;22)(p13;q12); carries t(3;3) and t(12;22) effecting MN1 and ETV6/MN1 rearrangements, respectively; precisely matches published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
