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| Cell line: | UPCI-SCC-016 |
| DSMZ no.: | ACC 884 |
| Species: | human (Homo sapiens) |
| Cell type: | squamous cell carcinoma |
| Origin: | sister cell line of UPCI-SCC-026 (DSMZ ACC 658); established in 1993 from a primary tumor of a well-differentiated squamous cell carcinoma of the tongue (stage T1NO)
of a 82-year-old non-smoking woman. Described to carry several chromosomal alterations including a 11q13 amplification and a mutation in TP53 (exon 8, R282W) |
| Reference(s): | 14735, 15061, 15176 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004). |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image |
| Medium: | 80-90% MEM (with Earle's salts) + 10-20% h.i. FBS + 1x MEM non-essential amino acids + 2 mM L-glutamine |
| Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 to 1:5 every 3-5 days using trypsin/EDTA (before trypsination wash 2x with PBS) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hrs |
| Harvest: | ca. 4-6 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
