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| Cell line: | UPCI-SCC-032 |
| DSMZ no.: | ACC 864 |
| Species: | human (Homo sapiens) |
| Cell type: | squamous cell carcinoma |
| Origin: | retromolar trigone squamous cell carcinoma established in 1993 from the primary tumor tissue (grade 2, T2N2b) of a 60-year-old man. Cells are described to carry a chromosomal 11q13 amplification. |
| Reference(s): | 15061, 14735, 15176 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004). |
| | DSMZ Cell Culture Data: |
| Morphology: | cells grow adherently, epithelioid-like in monolayers and before reaching confluency in a tri- to polyangular shape with prolonged cell extensions; image; image; image |
| Medium: | 80% MEM (with Earle's salts) + 20% h.i. FBS + non-essential amino acids + 2 mM L-glutamine |
| Subculture: | seed out at about 0.5- 1 x 106 cells/80 cm2; split semi-confluent or confluent culture 1:2 to 1:4 every 3-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | cell harvest of ca. 3-5 x 106 cells/80 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to make inquiries about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
