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| Cell line: | UPCI-SCC-090 |
| DSMZ no.: | ACC 670 |
| Species: | human (Homo sapiens) |
| Cell type: | squamous cell carcinoma |
| Origin: | established from an oral squamous cell carcinoma of a 44-year-old man; described in the literature to carry 100-150 copies of integrated human papilloma virus type 16 (HPV-16) |
| Reference(s): | 14735, 15061, 14530, 18143 |
| Biosafety level: | 2 |
| Risk assessment: | The cells are infected with human papilloma viruses (HPV) as detected by PCR. HPV sequences were also amplified applying PCR analysis on DNase I digested supernatant to detect DNase I protected active viruses. A transmission of HPV on handling the cells cannot be ruled out. The cell line was thus categorized biosafety level 2. |
| Permissions and restrictions: | A, B |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions. Requests for transfer to third parties and commercial entities may apply for licenses at the University of Pittsburgh, Office of Technology Management (Maria Vanegas; msv4@pitt.edu / 001-414-648-4004). |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial cells growing adherently as monolayer; image; image; image |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + 2 mM L-glutamine + non-essential amino acids |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:2 to 1:3 every 6-10 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 120-150 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV +, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
