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Cell line: | V-79 |
DSMZ no.: | ACC 335 |
Species: | Chinese hamster (Cricetulus griseus) |
Cell type: | lung fibroblasts |
Origin: | established from the lung tissue of a Chinese hamster in 1958 |
Reference(s): | 14504, 14542 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | fibroblast-like adherent cells growing in clumps; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 3 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 2-3 days; cells can be detached by pipetting with PBS or by mild treatment with trypsin/EDTA; after splitting, it is recommended to seed out the cells in fresh culture flasks as possibly the cells may not attach any longer in the old vessels |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 16-20 hours |
Harvest: | cell harvest of ca. 25 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species. |
Cytogenetics: | hamster highly rearranged near-diploid karyotype with 20% polyploidy - 22(20-24) - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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