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| Cell line: | WEHI-3B |
| DSMZ no.: | ACC 26 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | myelomonocytic leukemia |
| Origin: | macrophage-like cells derived from a BALB/c mouse treated for tumor induction; cells produce IL-3 |
| Reference(s): | 14877, 14878, 15054, 15222, 18195 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small, round, single cells growing adherently and partly in suspension; image; image; image; image; image |
| Medium: | 90-95% Iscove's MDM + 5-10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | split confluent culture 1:2 to 1:5 twice to three times weeky; cells are trypsin-sensitive, detach mechanically after loosening by incubating in PBS (with Ca2+ or Mg2+) for 10-15 min; seed out at ca. 0.2-0.5 x 105 cells/cm2; alternatively, cells can be detached using TrypLE Express (Invitrogen) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-30 hours |
| Harvest: | maximum cell harvest of about 1 x 106 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypotetraploid karyotype with 19% polyploidy - 73/74(70-79)<4n> - 2-3 centric fusion markers present |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
