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Cell line: | WMP-2 |
DSMZ no.: | ACC 453 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | lymphoblasts |
Origin: | established from spleen and lymph nodes of an adult Maloney murine leukemia virus (MoMuLV)-infected female WMP/WMP wild mouse; cells are described in the literature to carry nine pairs of metacentric Robertsonian translocation chromosomes containing 1 to 18; cells can be used for physical gene mapping by in situ hybridization in mice |
Reference(s): | 15079 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single round cells growing in suspension |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 50 µM mercaptoethanol |
Subculture: | split saturated culture at 1:3 to 1:5 every 2-4 days; seed out at ca. 2.5 x 105 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 2.0-2.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine karyotype with a preponderance of centric fusion markers - resembles published karyotype |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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