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| Cell line: | WMP-2 |
| DSMZ no.: | ACC 453 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | lymphoblasts |
| Origin: | established from spleen and lymph nodes of an adult Maloney murine leukemia virus (MoMuLV)-infected female WMP/WMP wild mouse; cells are described in the literature to carry nine pairs of metacentric Robertsonian translocation chromosomes containing 1 to 18; cells can be used for physical gene mapping by in situ hybridization in mice |
| Reference(s): | 15079 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round cells growing in suspension; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 50 µM mercaptoethanol |
| Subculture: | split saturated culture at 1:3 to 1:5 every 2-4 days; seed out at ca. 2.5 x 105 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-40 hours |
| Harvest: | cell harvest of ca. 2.0-2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine karyotype with a preponderance of centric fusion markers - resembles published karyotype |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
