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Cell line: | WSU-DLCL |
DSMZ no.: | ACC 779 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | established in 1988 from the malignant pleural fluid of a 32-year-old woman with diffuse large B cell lymphoma at terminal stage |
Reference(s): | 24246 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round cells growing mostly in clumps in suspension; image; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.2-0.3 x 106 cells/ml; optimal split ratio 1:2 - 1:3 every 3-4 days; cells start to proliferate after approximately one week |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48-72 hours |
Harvest: | maximum density at ca. 0.9 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD13 -, CD19 +, CD20 -, CD34 -, HLA-DR -, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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