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Cell line: | WSU-DLCL2 |
DSMZ no.: | ACC 575 |
Species: | human (Homo sapiens) |
Cell type: | B cell lymphoma |
Origin: | derived from the pleural effusion of a 41-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma non-cleaved type, intermediate grade - transformed from follicular small cleaved cell, low grade) at the status of relapse/progressive disease in 1990; cell line carries EZH2 Y641F mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 14353, 14777, 16553, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round and oval cells growing singly or in small clumps in suspension; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml;
split saturated culture 1:3-1:5 every 2-3 days; maintain at ca. 0.5-1.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | variable 40-60 hours |
Harvest: | cell harvest of ca. 1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Plasmocin, then negative in microbiological culture, and repeatedly in PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 -, CD 138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype - 47(43-48)<2n>XY, +7, +8, t(1;2)(p36;q37), del(3)(q25), t(4;14)(q27;q31), der(7)t(3;7)(q25;p13), i(7p), add(8)(q24)x2, del(13)(?q21?q22), t(14;18)(q32;q21), del(15)(q25q27) - carries t(14;18) effecting BCL2-IGH fusion - matches published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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