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Cell line: | XC |
DSMZ no.: | ACC 118 |
Species: | rat (Rattus norvegicus) |
Cell type: | sarcoma |
Origin: | the original tumor was induced in a Wistar rat by intramuscular injection of Rous sarcoma virus; cells have been used as indicator cells for detection of murine leukemia viruses |
Reference(s): | 14997, 14670, 14904 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-like, small cells growing as monolayers; image; image; image |
Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + non-essential amino acids |
Subculture: | split confluent culture 1:4 every 3 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Harvest: | about 1-2 x 107 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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