This online shop is using cookies to give you the best shopping experience. Thereby for example the session information or language setting are stored on your computer. Without cookies the range of the online shop's functionality is limited.
If you don't agree, please click here.
frog - South African clawed toad (Xenopus laevis) (Xenopus laevis)
heart endothelium cells
established from the embryonal (stage 52/53) normal heart endothelium (tadpole heart) of a South African clawed frog (Xenopus laevis Daudin) in 1979; cells were described in the literature to synthesize factor-VIII-related antigen (AHG-a.p.), fibronectin and laminin and to be tetraploid (72 +/-5, 2N = 36)
large epitheloid (low density monolayer), cobblestone with occasional piling-up (high density monolayer); image
90% Dulbecco's MEM (diluted with 20% distilled water) + 10% h.i. FBS + 20-30 mM HEPES
split confluent culture 1:2 every 10-12 days using trypsin/EDTA; avoid centrifugation, just dilute the harvested cells with medium and seed into a new flask; cells grow extremely slowly with a doubling time of about 10-12 days; thaw in cold water (10 °C), dilute with 10-fold volume of fresh culture medium, centrifuge gently (about 80 x g for 5 min)
keep at room temperature in closed flasks (with exposure to CO2)
ca. 10-12 days
cell harvest of about 2.5 x 106 cells/80 cm2
frozen with 90% complete medium + 10% glycerol (not DMSO)
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization assays
Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Xenopus laevis species.
PCR: SMRV -
Growing culture (please inquire for exact delivery time)