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Cell line: | XTH-2 |
DSMZ no.: | ACC 190 |
Species: | frog - South African clawed toad (Xenopus laevis) (Xenopus laevis) |
Cell type: | heart endothelium cells |
Origin: | established from the embryonal (stage 52/53) normal heart endothelium (tadpole heart) of a South African clawed frog (Xenopus laevis Daudin) in 1979; cells were described in the literature to synthesize factor-VIII-related antigen (AHG-a.p.), fibronectin and laminin and to be tetraploid (72 +/-5, 2N = 36) |
Reference(s): | 14935, 14933, 14934 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large epithelioid (low density monolayer), cobblestone with occasional piling-up (high density monolayer); image; image; image |
Medium: | 90% DMEM (diluted with 20% distilled water) + 10% h.i. FBS + 20-30 mM HEPES |
Subculture: | split confluent culture 1:2 every 10-12 days using trypsin/EDTA; avoid centrifugation, just dilute the harvested cells with medium and seed into a new flask; cells grow extremely slowly with a doubling time of about 10-12 days; thaw in cold water (10 °C), dilute with 10-fold volume of fresh culture medium, centrifuge gently (about 80 x g for 5 min) |
Incubation: | keep at room temperature in closed flasks (without exposure to CO2) |
Doubling time: | ca. 10-12 days |
Harvest: | cell harvest of about 2.5 x 106 cells/80 cm2 |
Storage: | frozen with 90% complete medium + 10% glycerol (not DMSO) |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Xenopus laevis species. |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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