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Cell line: | Y-79 |
DSMZ no.: | ACC 246 |
Species: | human (Homo sapiens) |
Cell type: | retinoblastoma |
Origin: | established from the primary tumor (right eye) of a 2-year-old girl in 1971; first RB cell line established and described to grow in soft agar and to initiate tumors in rabbits, partial deletion of the RB1 gene |
Reference(s): | 14888, 14757, 14758, 40297 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | cells grow in clusters in suspension; image; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 3.5 g/L glucose + 2 mM L-glutamine |
Subculture: | optimal split ratio at 1:2 two to three times per week; seed out at ca.0.25-0.5 x 106 cells/ml; after thawing, viability may drop below 50% temporarily |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 40 hours |
Harvest: | cell harvest of about 1.0-1.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 10% polyploidy - 47(45-48)<2n>XX, +mar, der(4)t(4;?6)(p15;p21.2), add(12)(q24.32), add(15)(q23), add(20)(q13) - large submetacentric marker with q-arm comprising HSR |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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