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Cell line: | YAC-1 |
DSMZ no.: | ACC 96 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | lymphoma |
Origin: | established by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse; cells were described in the literature to be sensitive to the cytotoxic activity of natural killer (NK) cells and are therefore often used as target cells in NK assays |
Reference(s): | 14660 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | round to oval, single or clustered cells in suspension; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 5 x 105 cells/ml, maintain at 0.3-2.0 x 106 cells/ml; cells grow very fast; split ratio of 1:5 to 1:10 |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 20 hours |
Harvest: | maximal density of about 3.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | hyperdiploid with 7% polyploidy - 41(38-42)<2n> - mouse chromosome morphology confirmed |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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