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established from ascites obtained during exploratory laparatomy of a 43-year-old man with pancreas carcinoma (terminal, no therapy) in 1993; cells were described as being dependent on autocrinely secreted IL-1alpha
cobblestone-like adherent cells growing in monolayers; image; image
90% RPMI 1640 + 15% h.i. FBS
seed out at ca. 1-2 x 106 cells/ 175 cm2; split culture up to 1:10 every 4 to 6 days prior to confluence (as the monolayer detaches upon reaching confluency) using trypsin/EDTA (incubate with trypsin/EDTA for 10-20 min); optimal density is at about 5 x 106 cells/175 cm2; it is recommended to add 10% of the spent medium to any fresh culture; after thawing cells may have a lag time of 1-2 days before reassuming growth
at 37 °C with 5% CO2
ca. 50-60 hours
cell harvest of ca. 2.0 x 107 cells/175 cm2
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
To inquire about expression of EpCAM and intermediate filaments, contact email@example.com.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
human near-triploid karyotype with 8% polyploidy - 66-71<3n>XXYY, +3, +7, +8, +9, +10, +14, -17, -18, -19, -20, -22, -22, -22, +3mar, der(1)del(1)(q23-25)inv(1)(p11q21), der(1;5)(q10;q10), add(3)(p11)/del(3)(p11p21)x2, del(5)(p11), add(6)(p21), del(7)(q32), add(9)(p1?), der(9;15)(q10;q10), der(10)t(10;11)(p11;q13)x2, del(12)(p12), add(13)(p11)x2, add(13)(p11), inv(16)(p13q11.2), del(18)(q21), add(20)(p11)/der(20)t(1;20)(p34;p11) - loss of 1p, 3p, 9p, 18q associated with pancreas carcinoma - matches published karyotype