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| Cell line: | YCUB-4R |
| DSMZ no.: | ACC 963 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established in 2007 from the peripheral blood of a 7-year-old boy with B-cell precursor acute lymphoblastic leukemia (ALL) at relapse; cells express a MEF2D exon5/BCL9 exon 9 fusion; sister cell line YCUB-4 established at diagnosis is available (DSMZ ACC 962) |
| Reference(s): | 40755, 18261 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small round cells growing singly in suspension; image; image |
| Medium: | 90% RPMI 1640 + 10% FBS |
| Subculture: | seed out at about 1 x 106 cells/ml; split
1:2 to 1:3 every 2-3 days; maintain at 0.5-1 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 48 hours |
| Harvest: | maximal density of ca. 1.5-2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3-, CD5+, CD10(+), CD13-, CD19+, CD20+, CD34-, CD37(+), CD38+, CD80-, CD138(+), HLA-DR+, cyCD79a+, sIgG-, sIgM+, cIgM+, sIg-kappa-, cIg-kappa-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the sister cell line YCUB-4 |
| Molec. Genetics: | fusion gene MEF2D::BCL9 was verified by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
