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| Cell line: | YCUB-5 |
| DSMZ no.: | ACC 964 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the bone marrow of a 12-year-old boy with BCR/ABL1-like (Ph-like) B-cell precursor acute lymphoblastoid leukemia (BCP-ALL) at diagnosis; carries a CRLF2-P2RY8 fusion; JAK2 R683I and KRAS G12D mutations are described |
| Reference(s): | 40754, 40755, 40756, 40795, 40796 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small, round cells growing singly in suspension; image; image |
| Medium: | 80% RPMI 1640 + 20% FBS |
| Subculture: | seed out at about 1 x 106 cells/ml; split
1:2 every 3 days; maintain between 0.5-2 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 72 hours |
| Harvest: | maximal density of ca. 2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3-, CD10+, CD13+, CD19+, CD20(+), CD34(+), CD37+, CD38+, CD80-, CD138-, HLA-DR+, sIgG-, sIgM-, sIg-kappa-, sIg-lambda-, cIgG-, cIgM-, cIg-kappa-, cIg-lambda-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | fusion gene P2RY8::CRLF2 was verified by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
