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established from the peripheral blood of a 46-year-old man with acute myeloid leukemia (AML M1) at diagnosis in 1994; cells were described in the literature to be constitutively cytokine-dependent and cytokine-responsive; cells carry the translocation t(16;21)(p11;q22) leading to FUS-ERG (TLS-ERG) fusion gene
single round cells growing in suspension, some cells are loosely adherent; image; image; image
60% RPMI 1640 + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 (DSMZ ACC 35) or 10 ng/ml G-CSF
seed out at ca. 0.5-1 x 106 cells/ml in 24-well plates; maintain at ca. 0.5-1 x 106 cells/ml; split saturated culture 1:2 every 3-4 days. CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus, the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines.
STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database.
human hyperdiploid karyotype with 4% polyploidy; -47(44-48)<2n>XY, +19, add(7)(q35), der(16)t(1;16)(q12;q12)t(16;21)(p11;q22), der(21)t(16;21)(p11;q22; carries complex t(16;21) effecting rearrangement of FUS (TLS) with ERG; matches published karyotype
expression of fusion gene FUS-ERG (TLS-ERG) confirmed by RT-PCR