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PLASMIDS | |
Name: | pGEc41 |
DSM No.: | 8829 dsm-8829 dsm8829 dsm 8829 pGEc41 |
History: | <- J. van Beilen, ETH Zürich |
Mol. weight: | 51.6 kb |
Remarks: | This alkBAC/alkR system is the first P. putida expression system described to be identically controlled in Pseudomonas and E. coli. Consists of the broad host range vector pLAFR1 (21.6 kb) and a 30 kb EcoRI fragment carrying the alkR locus and part of the alkBAC operon (alkBFGH and alkST genes). The alk regulon of the OCT plasmid is encoded by two distinct regions: alkBAC and alkR. While the plasmid-borne alk system for alkane hydroxylation and alkanol dehydrogenation is active in P. putida, it is active in E. coli only in fadR mutants with constitutive fatty acid degradation enzymes. Octane vapour is used as sole source of carbon and energy. AlkR locus is strictly necessary for expression of the 7.5 kb alkBAC operon. This expression is very efficient in E. coli. The cloned alk genes are covered by U.S. patent 5,173,428. |
Distributed in: | Escherichia coli K12 GEc137 |
Cultivation conditions: | Medium 694, 28°C |
Literature: | 6248, 6249, 6250 |
Risk group: | 1 (classification according to German TRBA) |
Restrictions: | German law regulating genetic engineering / Gentechnikgesetz), Category C Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
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Country: | country of origin unknown |
Date of sampling: | before 07.07.1995 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Supplied as: | Delivery form | | Organization type | | | | Profit | | Non-Profit | | | Freeze Dried | | 100,- € | | 100,- € | | Active culture on request | | 240,- € | | 240,- € | |
Price category 1 strain. Please see price list for freight and handling charges |
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