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In vivo recombinant plasmid; the 57 kb TOL fragment, including the 40 kb TOL metabolic region, was inserted into the complete SAL replicon at the position of SmaI-C within XhoI-B of SAL; pKF439 is self-transmissible to Pseudomonas putida and P. aeruginosa at high frequency; pKF439 encodes degradation of m-xylene, m-toluate and salicylate as sole carbon source.