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pME9 = pME305::Tn5-751; transposon-donor plasmid; the vector pME305 (44.4 kb) is a derivative of RP1, with a ts mutation in trfA; for insertional mutagenesis of Pseudomonas aeruginosa; the Tn5 derivative Tn5-751 (9.0 kb) was constructed.