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PLASMIDS | |
Name: | pMOB45 |
DSM No.: | 5593 dsm-5593 dsm5593 dsm 5593 pMOB45 |
Other collection no. or WDCM no.: | PC-V3069 |
History: | <- Phabagen |
Mol. weight: | 10.5 kb |
Marker: | Cmr, Tcr |
Remarks: | Derivative of the runaway-replication plasmid pKN402. General expression E. coli vector with the R1drd19 replicon. Amplifiable to high levels by heat induction: the copy number is then increased (> 100) when cultured at 40°C, roughly 75% of the total DNA is plasmid. This does not lead to inhibition of protein synthesis. |
Distributed in: | Escherichia coli K12 DB1443 C600 hsdR |
Cultivation conditions: | Medium 381, 37°C |
Literature: | 5182 |
Risk group: | 1 (classification according to German TRBA) |
Restrictions: | German law regulating genetic engineering / Gentechnikgesetz), Category C
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Country: | country of origin unknown |
Date of sampling: | before 15.06.1990 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Supplied as: | Delivery form | | Organization type | | | | Profit | | Non-Profit | | | Freeze Dried | | 100,- € | | 100,- € | | Active culture on request | | 240,- € | | 240,- € | |
Price category 1 strain. Please see price list for freight and handling charges |
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