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Agrobacterium binary vector ("plant gene vector cassette") with the characteristic property that Agrobacterium RK2 helper hosts not only maintain the vector but also mobilize it back to E. coli. Basic element of this cassette is a conditional mini-RK2 replicon, maintained and mobilized by trans-acting functions derived from RK2 and inserted into Agrobacterium hosts. Contains vir and tra functions of the Ti plasmid; self-transmissible.