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PLASMIDS | |
Name: | pNF3 |
DSM No.: | 4863 dsm-4863 dsm4863 dsm 4863 pNF3 |
Other collection no. or WDCM no.: | ATCC 37188 |
History: | <- E.C. Friedberg |
Mol. weight: | 8.1 kb |
Marker: | Apr and Kmr in E. coli, G418r and Ura+ in yeast |
Remarks: | pNF2 (DSM 4862) was converted into the integrating vector pNF3, a vector without a replicon for yeast. Since pNF3 carries the URA3 gene and the kamr gene from Tn903 as selection markers for Saccharomyces cerevisiae, DNA sequences containing a replicon for Saccharomyces cerevisiae can be selected. pNF3 has a unique BamHI cloning site flanked by SalI sites on both ends. |
Distributed in: | Escherichia coli K12 HB101 |
Cultivation conditions: | Medium 381, 37°C |
Literature: | 3553 |
Risk group: | 1 (classification according to German TRBA) |
Restrictions: | German law regulating genetic engineering / Gentechnikgesetz), Category C
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Country: | country of origin unknown |
Date of sampling: | before 16.03.1990 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Supplied as: | Delivery form | | Organization type | | | | Profit | | Non-Profit | | | Freeze Dried | | 100,- € | | 100,- € | | Active culture on request | | 240,- € | | 240,- € | |
Price category 1 strain. Please see price list for freight and handling charges |
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