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PLASMIDS | |
Name: | pRU669 |
DSM No.: | 4961 dsm-4961 dsm4961 dsm 4961 pRU669 |
History: | <- R. Schmitt |
Marker: | Cmr, incT, Kmr, tra+, ts |
Remarks: | The transposon Tn1725 (8.9 kb) was inserted in vivo into the conjugative Kmr ts plasmid Rts1 (Rts1::Tn1725); Tn1725 itself is a Cmr variant of Tn1722 obtained by ligation of a 3.3 kb HindIII fragment of R plasmid Sa that contains the cat gene into the single HindIII site of Tn1722; useful for mutagenesis in Escherichia coli; the large size of Rts1 (217 kb) prevents cotransformation of any Rts1::Tn replicons. |
Distributed in: | Escherichia coli K12 MM294 |
Cultivation conditions: | Medium 381, 30°C |
Literature: | 3774, 3780, 3784, 3792, 3796, 3799, 3801 |
Risk group: | 1 (classification according to German TRBA) |
Restrictions: | German law regulating genetic engineering / Gentechnikgesetz), Category C Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
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Country: | country of origin unknown |
Date of sampling: | before 16.03.1990 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Supplied as: | Delivery form | | Organization type | | | | Profit | | Non-Profit | | | Freeze Dried | | 100,- € | | 100,- € | | Active culture on request | | 240,- € | | 240,- € | |
Price category 1 strain. Please see price list for freight and handling charges |
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