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PLASMIDS | |
Name: | pRU670 |
DSM No.: | 4962 dsm-4962 dsm4962 dsm 4962 pRU670 |
History: | <- R. Schmitt |
Marker: | incT, Kmr, Tcr, tra+, ts |
Remarks: | The transposon Tn1731 (7.8 kb) was inserted in vivo into the conjugative Kmr ts plasmid Rts1 (Rts1::Tn1731); Tn1731 itself is a Tcr variant of Tn1722, an in vitro recombinant of Tn1722 and a 2.4 kb EcoRI fragment carrying the tet genes of Tn1721; the EcoRI fragment was derived from plasmid pJOE452; useful for mutagenesis in Escherichia coli; the large size of Rts1 (217 kb) prevents cotransformation of any Rts1::Tn replicons. |
Distributed in: | Escherichia coli K12 MM294 |
Cultivation conditions: | Medium 381, 30°C |
Literature: | 3774, 3784, 3792, 3796, 3799, 3802 |
Risk group: | 1 (classification according to German TRBA) |
Restrictions: | German law regulating genetic engineering / Gentechnikgesetz), Category C Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
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Country: | country of origin unknown |
Date of sampling: | before 16.03.1990 |
Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
Supplied as: | Delivery form | | Organization type | | | | Profit | | Non-Profit | | | Freeze Dried | | 100,- € | | 100,- € | | Active culture on request | | 240,- € | | 240,- € | |
Price category 1 strain. Please see price list for freight and handling charges |
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