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Apr, incP, Kmr, Smr (not expressed in E. coli host), Tcr, tra+, trfAts
The transposon Tn1733 (8.9 kb) was inserted in vivo into the ts conjugative broad-host-range R plasmid pME305 (Apr, Tcr) (pME305::Tn1733); Tn1733 itself is a Kmr (Smr) variant of Tn1722, obtained by ligation of a 3.3 kb HindIII fragment that comprises the central region of Tn5 into the single HindIII site of Tn1722; the cloned fragment encodes Kmr, ble and str (str is cryptic in Escherichia coli, but is expressed in different nonenteric bacteria); useful for mutagenesis in various Gram-negative bacteria.