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| PLASMIDS | |
| Name: | pRU672 |
| DSM No.: | 4965 dsm-4965 dsm4965 dsm 4965 pRU672 |
| History: | <- R. Schmitt |
| Marker: | Apr, incP, Kmr, Smr (not expressed in E. coli host), Tcr, tra+, trfAts |
| Remarks: | The transposon Tn1733 (8.9 kb) was inserted in vivo into the ts conjugative broad-host-range R plasmid pME305 (Apr, Tcr) (pME305::Tn1733); Tn1733 itself is a Kmr (Smr) variant of Tn1722, obtained by ligation of a 3.3 kb HindIII fragment that comprises the central region of Tn5 into the single HindIII site of Tn1722; the cloned fragment encodes Kmr, ble and str (str is cryptic in Escherichia coli, but is expressed in different nonenteric bacteria); useful for mutagenesis in various Gram-negative bacteria. |
| Distributed in: | Escherichia coli K12 MM294 |
| Cultivation conditions: | Medium 381, 30°C |
| Literature: | 3775, 3784, 3791, 3792, 3799 |
| Risk group: | 1 (classification according to German TRBA) |
| Restrictions: | German law regulating genetic engineering / Gentechnikgesetz), Category C
Material transfer agreement, Category DCommercial use and transfer to third parties is completely excluded for this strain.
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| Country: | country of origin unknown |
| Date of sampling: | before 16.03.1990 |
| Nagoya Protocol Restrictions: | There are NO known Nagoya protocol restrictions for this strain. |
| Supplied as: | | Delivery form | | Organization type | | | | | Profit | | Non-Profit | | | | Freeze Dried | | 100,- € | | 100,- € | | | Active culture on request | | 240,- € | | 240,- € | |
Price category 1 strain.
Please see price list for freight and handling charges |
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