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Expression vector; cloning vector with multiple restriction enzyme linker; derived from pBR322 and M13mp9-vector; pUC9 allows direct selection of cloned DNA because of α complementation; allows using the same primer as for the M13 system; pUC9 allows to clone double-digested restriction fragments separately in both orientation with respect to the lac promoter; replicon: pMB1; not self-transmissible.