| | |
|---|
| Cell line: | CHO-K1 |
| DSMZ no.: | ACC 110 |
| Species: | Chinese hamster (Cricetulus griseus) |
| Cell type: | ovary cells |
| Origin: | subclone from parental CHO cell line with distinctive karyotype; CHO cell line was initiated from an ovary biopsy of an adult Chinese hamster in 1957 |
| Reference(s): | 14649, 14860, 14592, 40427 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, fibroblastoid cells, may be induced to undergo additional differentiation; image; image; image; image; image; image |
| Medium: | 90% Ham's F12 + 10% h.i. FBS |
| Subculture: | seed out about 105 - 106 cells in an 80 cm2 flask; split confluent cultures 1:3 to 1:10 using trypsin/EDTA once or twice a week |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 24 hours |
| Harvest: | about 10 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species. |
| Cytogenetics: | near diploid karyotype with 12% polyploidy - 22(20-23) - exhibits distinctive CHO karyology |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
