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Cell line: | CHO-K1 |
DSMZ no.: | ACC 110 |
Species: | Chinese hamster (Cricetulus griseus) |
Cell type: | ovary cells |
Origin: | subclone from parental CHO cell line with distinctive karyotype; CHO cell line was initiated from an ovary biopsy of an adult Chinese hamster in 1957 |
Reference(s): | 14649, 14860, 14592, 40427 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent, fibroblastoid cells, may be induced to undergo additional differentiation; image; image; image; image; image; image |
Medium: | 90% Ham's F12 + 10% h.i. FBS |
Subculture: | seed out about 105 - 106 cells in an 80 cm2 flask; split confluent cultures 1:3 to 1:10 using trypsin/EDTA once or twice a week |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 24 hours |
Harvest: | about 10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Cricetulus griseus species. |
Cytogenetics: | near diploid karyotype with 12% polyploidy - 22(20-23) - exhibits distinctive CHO karyology |
Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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