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Cell line: | MN-60 |
DSMZ no.: | ACC 138 |
Species: | human (Homo sapiens) |
Cell type: | B cell leukemia |
Origin: | established in 1981 from the peripheral blood of a 20-year-old man with acute B cell leukemia of Burkitt's type (ALL FAB L3) in partial remission |
Reference(s): | 14899, 40797 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round to polygonal cells in suspension; image; image; image; image; image |
Medium: | 90% Ham's F10 + 10% h.i. FBS |
Subculture: | maintain at 0.2-0.5 x 106 cells/ml; seed out at ca. 0.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48-60 hours |
Harvest: | maximal density at 1.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near diploid karyotype - 46(45-47)<2n>XY, dup(1)(q21q41), del(6)(q21), t(8;14)(q24;q32), i(13q) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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