| | |
|---|
| Cell line: | PC-12 |
| DSMZ no.: | ACC 159 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | adrenal pheochromocytoma |
| Origin: | established from a transplantable rat adrenal pheochromocytoma in 1976; cells were described in the literature to synthesize catecholamines (dopamine, norepinephrine) and in response to nerve growth factor (NGF) a neuronal phenotype could be induced reversibly |
| Reference(s): | 14576, 14716 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small cells growing in clumps in suspension, adhering poorly to plastic; image; image; image; image |
| Medium: | 85% RPMI 1640 + 10% horse serum + 5% h.i. FBS |
| Subculture: | split saturated culture 1:2 to 1:3 every 3-4 days; cells grow in suspension without collagen coating; with collagen coating (Collagen R- Roche at 30 µg/ml) cells grow adherent, faster and to a higher density; the adherent cells may be detached mechanically without trypsin; for single cells the suspension should be passed several times through a syringe with a 22-gauge needle; seed out at ca. 5-15 x 106 cells/25 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | about 1-2 x 106 cells/ml or 30 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat hypodiploid karyotype with 20% polyploidy - 39(38-40)<2n> |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
