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Cell line: | PA-TU-8988T |
DSMZ no.: | ACC 162 |
Species: | human (Homo sapiens) |
Cell type: | pancreas adenocarcinoma |
Origin: | established in 1985 from the liver metastasis of a primary pancreatic adenocarcinoma from a 64-year-old woman; described to carry mutations in KRAS, SMAD4 and TP53;
sister cell line of PA-TU-8988S (DSMZ ACC 204) |
Reference(s): | 14508, 40710, 40728 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent cells growing in monolayers; image; image; image; image; image |
Medium: | 90% DMEM + 5% h.i. FBS + 5% horse serum + 2 mM L-glutamine (some researcher cultivate also with horse serum) |
Subculture: | seed out at ca. 0.5 x 106 cells/80 cm2; split confluent culture 1:5 to 1:10 every 3-5 days using trypsin/EDTA |
Incubation: | at 37 °C with 5-10% CO2 |
Doubling time: | about 25-30 hours |
Harvest: | cell harvest of about 20 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-triploid karyotype with 2% polyploidy - 68(63-70)<3n>XXX, -4, +6, +6, +8, +11, -15, -16, -17, -19, +20, -21, -22, +mar, der(1)t(1;?)(p11;?), i(6p), del(6)(q23)x2, der(8)t(8;?)(q24.3;?), der(8)t(8;?)(p11;?)x2, der(11)t(11;?)(p13;?)x2, del(12)(p12.3), dup(14(q11q12), del(17)(p12), der(17)t(17;?)(p13;?) - sideline with additional der(3)t(3;?)(q11;?) - 1p11 and 8q24 breakpoints previously reported in pancreatic cancer |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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