| |
---|
Cell line: | MSTO-211H |
DSMZ no.: | ACC 390 |
Species: | human (Homo sapiens) |
Cell type: | mesothelioma |
Origin: | established from the pleural effusion of a 62-year-old man with mesothelioma (malignant biphasic) who did not have any prior therapy |
Reference(s): | 14382 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent fibroblast-like cells growing as monolayer; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | split confluent culture 1:5 every 3 days using trypsin/EDTA; seed out at ca. 2-3 x 106 cells/80 cm2 in 10 ml medium |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | cell harvest of ca. 15 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypertriploid karyotype - 79(77-80)<3n>XYY/XXYY, +2, +4, +7, +7, +7, -10, +12, +13, +14, -15, -15, +16, +16, +16, -17, +18, -22, +1-2mar, 1-20dmin - add(1)(p35-36), add(1)(p34), der(4)t(4;17)(p14;q21)x2, del(6)(q25)x2, del(7)(q11), del(7)(q21.2)x2, inv(9)(p24q34), del(10)(q24), add(14)(p11), add(15)(p11), i?dic(16)(q10)x2 - sideline with der(6;9)(p10;q10) - high 7p copy number associated with poor prognosis mesothelioma - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |