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| Cell line: | BTI-EAA |
| DSMZ no.: | ACC 176 |
| Species: | insect - Estigmene acrea (Estigmene acrea) |
| Cell type: | |
| Origin: | established from the larvae of the Acrea Moth (Estigmene acrea; family Arctiidae, order Lepidoptera) (Baerenspinner) |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | very large cells growing in suspension; most cells are round to oval, 10% have long processes and are spindle shaped; image; image; image; image; image; image |
| Medium: | 80% TC 100 medium + 20% h.i. FBS |
| Subculture: | cells are difficult to culture and grow slowly! split saturated culture 1:4 to 1:5 every 4-5 days |
| Incubation: | no CO2 at room temperature (20-25 °C) |
| Harvest: | cell harvest of about 0.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Estigmene acrea species. |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
