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Cell line: | BTI-EAA |
DSMZ no.: | ACC 176 |
Species: | insect - Estigmene acrea (Estigmene acrea) |
Cell type: | |
Origin: | established from the larvae of the Acrea Moth (Estigmene acrea; family Arctiidae, order Lepidoptera) (Baerenspinner) |
Reference(s): | not published |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | very large cells growing in suspension; most cells are round to oval, 10% have long processes and are spindle shaped; image; image; image; image; image; image |
Medium: | 80% TC 100 medium + 20% h.i. FBS |
Subculture: | cells are difficult to culture and grow slowly! split saturated culture 1:4 to 1:5 every 4-5 days |
Incubation: | no CO2 at room temperature (20-25 °C) |
Harvest: | cell harvest of about 0.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Estigmene acrea species. |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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