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Cell line: | SUP-T1 |
DSMZ no.: | ACC 140 |
Species: | human (Homo sapiens) |
Cell type: | T cell lymphoma |
Origin: | established from the pleural effusion of an 8-year-old boy with T-lymphoblastic lymphoma in relapse |
Reference(s): | 14970 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round cells in suspension; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml; maintain at 0.3-1.0 x 106 cells/ml; split ratio of 1:2 to 1:3 every 2-3 days; after thawing the viability may drop to less than 50%, but the cells recover during the following 4-6 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30 hours |
Harvest: | maximal density of 1.5-2.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, thereafter negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | cyCD3 +, CD4 +, CD5 +, CD6 +, CD7 +, CD8 +, CD13 -, CD19 -, CD34 -, TCRalpha/beta -, TCRgamma/delta -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotetraploid karyotype with 1.8% polyploidy - 85<4n>XXX/XXX?Y, -8, -8, -9, -12, inv(2)(p22q11)x2, t(2;?20)(p13;?p11), del(4)(q31q35), del(6)(q25)x2, add(7)(q32), add(9)(q34)x2, inv(14)(q11q32)x2 - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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