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| Cell line: | COLO-680N |
| DSMZ no.: | ACC 182 |
| Species: | human (Homo sapiens) |
| Cell type: | esophageal squamous cell carcinoma |
| Origin: | derived from the esophagus squamous cell carcinoma of a 58-year-old woman in 1985 via passage through a nude mouse |
| Reference(s): | 40503 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epitheloid cells growing in monolayers; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent cultures 1:2 to 1:3 every 2-3 days using trypsin/EDTA; |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 60-70 hours |
| Harvest: | cell harvest of about 10-12 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human highly rearranged hyperdiploid/hypotriploid karyotype with 13.5% polyploidy - 53-58<2n>X, -X, +1, +1, -3, -5, -5, +6, +7, +12, -13, -14, -14, -16, -16, -18, -20, -21, -22, +16mar - add(1)(p35)x1-2, add(1)(q11)x1-2, add(1)(p11), add(3)(p11), add(4)(q35), add(7)(q35)x1-2, add(7)(p22)del(7)(q11q21), der(9)t(5;9)(q14;p11), del(11)(p11), add(12)(q24), add(19)(p13), add(19)(q13) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
