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Cell line: | SH-SY5Y |
DSMZ no.: | ACC 209 |
Species: | human (Homo sapiens) |
Cell type: | neuroblastoma |
Origin: | clonal subline of the neuroepithelioma cell line SK-N-SH that had been established in 1970 from the bone marrow biopsy of a 4-year-old girl with metastatic neuroblastoma (supposedly N-type and described to carry a ALK mutation). Widely used and extensively characterized cell line; listed in the CCLE (ref 18232) |
Reference(s): | 14388, 14638, 14901, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epithelial-/neuronal-like elongated cells growing as monolayer and in cell clusters; cells do not grow to complete confluency; image; image; image |
Medium: | 80-85% DMEM + 15-20% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells in a T75 flask; split culture 1-2 times a week using trypsin/EDTA; selection of neuronal-like cells may be favored by reduction of glutamine (1 mM), passaging of semi- or half-confluent cultures; short-time trypsin exposure preferentially detaches the epithelial-like cells |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50-60 hours |
Harvest: | cell harvest of about 15 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human near-diploid karyotype with 1.8% polyploidy - 46/47(42-48)<2n>X/XX, +7, ins(1)(q32q11q43), add(9)(q34), der(22)t(?17;22)(q22;q13) - resembles published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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