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Cell line: | COLO-206F |
DSMZ no.: | ACC 21 |
Species: | human (Homo sapiens) |
Cell type: | colon carcinoma |
Origin: | established from the ascites fluid of a 71-year-old man with colon carcinoma in 1975; The cell line was established from the same patient as the cell lines COLO-201 and COLO-205 |
Reference(s): | not published |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | mostly round cells; about 80% are adherent cells; about 2-8% spindle-shaped cells; some giant cells; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-3 x 106 cells/80 cm2; split confluent cultures about 1:6 once a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 2-3 days |
Harvest: | about 7-10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 10% polyploidy - 72(66-77)<2n>XX, -Y, +1, +3, +3, +5, -6, +7, -8, +9, -10, -13, -18, +20, -21, +3mar, i(1q), der(1)t(1;18)(q11;p11), der(3) t(3;?)(p21;?)x2, del(10)(q23.3), der(12)t(3;12)(q11;p13) - a small supernumerary centric fragment or ring present in 18% of cells |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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