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Cell line: | CII |
DSMZ no.: | ACC 773 |
Species: | human (Homo sapiens) |
Cell type: | chronic B cell leukemia |
Origin: | established in 1979 from Epstein-Barr virus (EBV) immortalized neoplastic lymphocytes of a 47-year-old woman with chronic lymphocytic leukemia (CLL, Rai II stage at diagnosis); the CI cell line - formerly thought of as a B-lymphoblastoid sister - is now deemed a subclone of the putatively oligoclonal CII cell line; cells express stereotyped subset 5 BCR |
Reference(s): | 19602, 19603, 19604, 19172 |
Biosafety level: | 1 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 was negative by western blot in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1.
According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | semi-adherent cells show round or ovoid morphology growing in clusters; long cultivation period leads to attachment of the cells and epitheloid morphology; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/25 cm2; optimal split ratio 1:2 - 1:4 every 2-4 days; adherent cells can be detached by tapping (or trypsination) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 40-60 hours |
Harvest: | cell harvest of ca. 4 x 106 cells/25 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD5 +, CD10 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypertetraploid karyotype; 93-97<4n>XXXXX, +X, +12, der(1)t(1;20)(p36;p11), der(12)t(12;14)(p11;q11), add(17)(p11)x2, del(16)(q12); sdl with additional numerical and structural aberrations; some resemblance to published karyotype |
Viruses: | PCR: EBV +, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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