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Cell line: | EFE-184 |
DSMZ no.: | ACC 230 |
Species: | human (Homo sapiens) |
Cell type: | endometrium carcinoma |
Origin: | established from the ascitic fluid of a 69-year-old woman with endometrial carcinoma in relapse in 1985 |
Reference(s): | not published |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing in monolayers; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out about 1 x 106/175 cm2; split confluent culture 1:4 once a week using trypsin/EDTA; cells grow very slowly |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 90 hours |
Harvest: | cell harvest of about 4-5 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypertriploid karyotype with 1-2 supernumerary dm in most cells and 23% polyploidy - 80(72-81)<3n>XX, -X, +1, +2, -4, -5, +6, +8, +10, -13, +17, +19, +20, +21, +6mar - der(1)t(1;?)(p11;?)x2, del(2)(q24)x2, der(2)t(2;3)(q11;p11), del(12)(p13), der(16)t(16;?)(q21/23;?), der(17)t(7;17)(q11;p11), add(22)(p11)x2 |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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