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Cell line: | BEN |
DSMZ no.: | ACC 254 |
Species: | human (Homo sapiens) |
Cell type: | lung carcinoma |
Origin: | established from the supraclavicular tumor cells-containing lymph node of a 71-year-old man with poorly differentiated carcinoma of the lung, classified as large cell carcinoma; cells cary a complex karyotype; listed in the CCLE (ref 18232) |
Reference(s): | 14507, 40412, 40405, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent, epitheloid cells growing in monolayers; image; image; image; image |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2 flask; split confluent culture 1:3 every 3-4 days using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | about 40-50 hours |
Harvest: | cell harvest of about 20 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human highly rearranged flat-moded near-tetraploid karyotype - 82-92<4n>X/XY, -X, -Y, -Y, -2, -2, -3, -3, -4, -4, -5, +6, +6, +7, -8, -8, -9, -10, -13, -13, -14, -16, -16, -17, -18, -18, -19, -21, -21, -21, -22, +18mar - del(1)(q22)x2, add(1)(p11), i(2q), i(4q), add(5)(p11), del(6)(q13)x3, del(6)(p21.2), der(7)(t(7;12)(p11;q11), del(7)(q21)x2, i(7p), del(8)(p21), del(9)(p12), del(10)(q24), del(11)(p11)x2, add(12)(q24), i(12q), del(?12)(q14), add(14)(p11), add(18)(q21/23), add(19)(p13) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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